Cooperative Unfolding of Residual Structure in Heat Denatured Proteins by Urea and Guanidinium Chloride
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منابع مشابه
Cooperative Unfolding of Residual Structure in Heat Denatured Proteins by Urea and Guanidinium Chloride
The denatured states of proteins have always attracted our attention due to the fact that the denatured state is the only experimentally achievable state of a protein, which can be taken as initial reference state for considering the in vitro folding and defining the native protein stability. It is known that heat and guanidinium chloride (GdmCl) give structurally different states of RNase-A, l...
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A number of very careful studies have been made in recent years of the thermal transitions which small globular proteins undergo at low pH. These transitions reflect the destruction of the ordered conformation of the native protein, and the products of the transition have the properties of highly disordered polypeptide chains. The principal objective of such studies has, however, been to determ...
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Small-angle neutron scattering measurements were performed on two very different proteins unfolded by guanidinium chloride. It is shown that the neutron spectra given by unfolded phosphoglycerate kinase and p-casein are similar to those of excluded volume homopolymer chains. INTRODUCTION A functional native protein generally adopts a specific three-dimensional structure that is determined by it...
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The pressure-induced unfolding of lysozyme was investigated in an aqueous guanidinium chloride solution by means of ultraviolet spectroscopy. Assuming a two-state transition model, volume changes were calculated from the slope of free energy vs. pressure plots over a temperature range of 10 to 60 degrees C. Between 25 and 60 degrees C, almost constant volume changes were observed in the transit...
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Horse ferricytochrome c (cyt c) undergoes exchange of one of its axial heme ligands (Met-80) for one or more non-native ligands under denaturing conditions. We have used (1)H NMR spectroscopy to detect two conformations of paramagnetic cyt c with non-native heme ligation through a range of urea concentrations. One non-native form is an equilibrium unfolding intermediate observed under partially...
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ژورنال
عنوان ژورنال: PLOS ONE
سال: 2015
ISSN: 1932-6203
DOI: 10.1371/journal.pone.0128740